The behaviors of human mesenchymal stem cells on the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) membranes

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a potential material for tissue engineering applications because of its excellent biodegradable and biocompatible properties. The aim of this study is to present a close picture of the interaction between human mesenchymal stem cells (hMSCs) and the surface characteristics of PHBHHx membranes. The surface characteristics of PHBHHx membranes were created by using compressionmolding, solvent-casting, and electrospinning methods. The behaviors of the hMSCs performed differently on those membranes. The highly porous electrospun PHBHHx membranes are better substrates for culturing hMSCs as they can keep higher cell viability than the compression-molded PHBHHx membranes. Aggregation and cluster formation of hMSCs were found only on the cast PHBHHx membranes.

The surface characteristics of the membranes, created by using compression-molding, solventcasting and electrospinning methods, influenced the activities of hMSCs. HMSCs aggregated and form clusters on the cast PHBHHx membrane,