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Transmembrane pressure was the major parameter responsible for the fouling layer formation, while the increase of crossflow velocity and the addition of enzyme contributed to diminish the polarized layer. At high transmembrane pressure values, enzyme treatment had more influence in polarized layer resistance. In the conditions used in this study, the best condition for umbu microfiltration is low transmembrane pressure, high crossflow velocity and enzyme treatment. ±E ±v ±P ±E ±v ±P ±E ±v ±P +E+v +P –E +v +P +E –v +P –E –v +P –E +v –P +E +v –P 0.0 +E –v –P 1.0 –E –v –P Resistance values 10–12 (m–1) 8.0 Fig. 1. Flux resistances, where “–, + and ±” mean the minimum condition, maximum and intermediate conditions. RP, RF and RM refers to resistances due to polarized layer, fouling and membrane, respectively.